Human PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit

Product Overview

• Article Name: Human PTGS2/COX-2(Prostaglandin Endoperoxide Synthase 2) ELISA Kit
• Biozol Catalog Number: EBT-ELK2096
• Supplier Catalog Number: ELK2096
• Alternative Catalog Number: EBT-ELK2096-96, EBT-ELK2096-48, EBT-ELK2096-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Human
• Alternative Names: PGG/HS, PGHS2, PHS2, HCox2, COX2, Cyclooxygenase 2, Prostaglandin G/H Synthase And Cyclooxygenase, Prostaglandin H2 synthase 2, PTGS2, Prostaglandin Endoperoxide Synthase 2

Product Properties

• Concentration: 20 ng/mL
• Range: 0.32-20 ng/mL
• Sensitivity: 0.125 ng/mL
• UniProt: P35354
• Samples: serum, plasma, tissue homogenates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,Tumor immunity,Infection immunity,Reproductive science,Pulmonology,Gastroenterology,Hormone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTGS2/COX-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTGS2/COX-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTGS2/COX-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PTGS2/COX-2 in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK2096