Human Ab1-42(Amyloid Beta Peptide 1-42) Microsample ELISA Kit

Catalog Number: EBT-ELK2100MS
Article Name: Human Ab1-42(Amyloid Beta Peptide 1-42) Microsample ELISA Kit
Biozol Catalog Number: EBT-ELK2100MS
Supplier Catalog Number: ELK2100MS
Alternative Catalog Number: EBT-ELK2100MS-96, EBT-ELK2100MS-48, EBT-ELK2100MS-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: Amyloid beta 42(ABeta 42), amyloid beta peptide 1-42, Abeta1-42,amyloid betapeptide 1-42
Concentration: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sensitivity: 5.16 pg/mL
Samples: serum, plasma and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2100MS