Human BAFF/CD257(B-Cell Activating Factor) ELISA Kit

Product Overview

• Article Name: Human BAFF/CD257(B-Cell Activating Factor) ELISA Kit
• Biozol Catalog Number: EBT-ELK2145
• Supplier Catalog Number: ELK2145
• Alternative Catalog Number: EBT-ELK2145-96, EBT-ELK2145-48, EBT-ELK2145-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Human
• Alternative Names: CD257, TNFSF13B, BLYS, TALL1, THANK, TNFSF20, ZTNF4, B lymphocyte stimulator, Tumor Necrosis Factor Ligand Superfamily Member 13B, Dendritic cell-derived TNF-like, BAFF

Product Properties

• Concentration: 5000 pg/mL
• Range: 78.13-5000 pg/mL
• Sensitivity: 28 pg/mL
• UniProt: Q9Y275
• Samples: serum, plasma, tissue homogenates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: CD & Adhesion molecule,Tumor immunity,Infection immunity,Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK2145