Rat PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit
Biozol Catalog Number:
EBT-ELK2409
Supplier Catalog Number:
ELK2409
Alternative Catalog Number:
EBT-ELK2409-96, EBT-ELK2409-48, EBT-ELK2409-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Rat
Alternative Names:
P2CP, C-Propeptide Of Type II Procollagen, Procollagen II Carboxy Terminal Propeptide
Concentration:
2000 pg/mL
Range:
31.25-2000 pg/mL
Sensitivity:
12.3 pg/mL
Samples:
Serum, plasma and other biological fluids
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Infection immunity,Bone metabolism,Rheumatology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PIICP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PIICP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat PIICP in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2409
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