Chicken HSPb2(Heat Shock Protein Beta 2) ELISA Kit
Biozol Catalog Number:
EBT-ELK2431
Supplier Catalog Number:
ELK2431
Alternative Catalog Number:
EBT-ELK2431-96, EBT-ELK2431-48, EBT-ELK2431-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Gallus
Alternative Names:
HSP-B2, MKBP, HSP27, Heat Shock 27kDa Protein 2, DMPK-binding protein
Concentration:
1000 pg/mL
Range:
15.63-1000 pg/mL
Sensitivity:
5.5 pg/mL
Samples:
Tissue homogenates and other biological fluids.
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Tumor immunity,Developmental science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken HSPb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken HSPb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken HSPb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Chicken HSPb2 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2431
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