Rat PIINP(Procollagen II N-Terminal Propeptide) ELISA Kit
Biozol Catalog Number:
EBT-ELK2574
Supplier Catalog Number:
ELK2574
Alternative Catalog Number:
EBT-ELK2574-96, EBT-ELK2574-48, EBT-ELK2574-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Rat
Alternative Names:
P2NP, N-Propeptide Of Type II Procollagen, Procollagen II Amino Terminal Propeptide
Concentration:
20 ng/mL
Range:
0.32-20 ng/mL
Sensitivity:
0.118 ng/mL
Samples:
Serum, plasma, tissue homogenates and other biological fluids
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Bone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PIINP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PIINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat PIINP in the samples is then determined by comparing the OD of the samples to the standard curve
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