Mouse ENA78(Epithelial Neutrophil Activating Peptide 78) Microsample ELISA Kit

Catalog Number: EBT-ELK2652MS
Article Name: Mouse ENA78(Epithelial Neutrophil Activating Peptide 78) Microsample ELISA Kit
Biozol Catalog Number: EBT-ELK2652MS
Supplier Catalog Number: ELK2652MS
Alternative Catalog Number: EBT-ELK2652MS-96, EBT-ELK2652MS-48, EBT-ELK2652MS-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Mouse
Alternative Names: CXCL5, SCYB5, Chemokine C-X-C-Motif Ligand 5, Small Inducible Cytokine Subfamily B(Cys-X-Cys),Member 5, Neutrophil-activating peptide ENA-78
Concentration: 4000 pg/mL
Range: 62.5-4000 pg/mL
Sensitivity: 24 pg/mL
Samples: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ENA78. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ENA78. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ENA78, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse ENA78 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK2652MS