Rat HBEGF(Heparin Binding Epidermal Growth Factor Like Growth Factor) ELISA Kit

Product Overview

• Article Name: Rat HBEGF(Heparin Binding Epidermal Growth Factor Like Growth Factor) ELISA Kit
• Biozol Catalog Number: EBT-ELK2766
• Supplier Catalog Number: ELK2766
• Alternative Catalog Number: EBT-ELK2766-96, EBT-ELK2766-48, EBT-ELK2766-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Rat
• Alternative Names: HB-EGF, DTR, DTS, DTSF, HEGFL, Diphtheria Toxin Receptor, Proheparin-binding EGF-like growth factor, Diphtheria toxin receptor

Product Properties

• Concentration: 1000 pg/mL
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.3 pg/mL
• UniProt: Q06175
• Samples: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HBEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HBEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HBEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat HBEGF in the samples is then determined by comparing the OD of the samples to the standard curve

Images

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