Human S100(S100 Calcium Binding Protein) ELISA Kit

Product Overview

• Article Name: Human S100(S100 Calcium Binding Protein) ELISA Kit
• Biozol Catalog Number: EBT-ELK2904
• Supplier Catalog Number: ELK2904
• Alternative Catalog Number: EBT-ELK2904-96, EBT-ELK2904-48, EBT-ELK2904-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Human
• Alternative Names: S100-A1, S100A1, S100-Alpha, S-100 Protein, S-100 protein subunit alpha, S-100 protein alpha chain

Product Properties

• Concentration: 100 ng/mL
• Range: 1.57-100 ng/mL
• Sensitivity: 0.64 ng/mL
• UniProt: B2R5H0
• Samples: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human S100 in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK2904