Rabbit NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Product Overview

• Article Name: Rabbit NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
• Biozol Catalog Number: EBT-ELK2957
• Supplier Catalog Number: ELK2957
• Alternative Catalog Number: EBT-ELK2957-96, EBT-ELK2957-48, EBT-ELK2957-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Rabbit
• Alternative Names: NT-Pro-BNP, , N-BNP, ANFB_HUMAN, BNP(1-32), BNP(5-29), BNP-32, Gamma-brain natriuretic peptide, nppb

Product Properties

• Concentration: 2000 pg/mL
• Range: 31.25-2000 pg/mL
• Sensitivity: 12.5 pg/mL
• Samples: Serum, plasma, tissue homogenates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Endocrinology,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rabbit NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK2957