Human ITIH4(Inter Alpha-Globulin Inhibitor H4) ELISA Kit

Catalog Number: EBT-ELK3097
Article Name: Human ITIH4(Inter Alpha-Globulin Inhibitor H4) ELISA Kit
Biozol Catalog Number: EBT-ELK3097
Supplier Catalog Number: ELK3097
Alternative Catalog Number: EBT-ELK3097-96, EBT-ELK3097-48, EBT-ELK3097-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: IHRP, PK120, H4P, ITIHL1, Plasma Kallikrein-Sensitive Glycoprotein, Plasma kallikrein sensitive glycoprotein 120, Inter-alpha-trypsin inhibitor family heavy chain-related protein
Concentration: 25 ng/mL
Range: 0.4-25 ng/mL
Sensitivity: 0.17 ng/mL
Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITIH4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITIH4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITIH4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human ITIH4 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3097