Human GPR37(G Protein Coupled Receptor 37) Microsample ELISA Kit

Catalog Number: EBT-ELK3116MS
Article Name: Human GPR37(G Protein Coupled Receptor 37) Microsample ELISA Kit
Biozol Catalog Number: EBT-ELK3116MS
Supplier Catalog Number: ELK3116MS
Alternative Catalog Number: EBT-ELK3116MS-96, EBT-ELK3116MS-48, EBT-ELK3116MS-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: EDNRBL, PAELR, hET(B)R-LP, Endothelin Receptor Type B-Like, Parkin-associated endothelin receptor-like receptor, Prosaposin receptor GPR37
Concentration: 10 ng/mL
Range: 0.16-10 ng/mL
Sensitivity: 0.059 ng/mL
UniProt: O15354
Samples: tissue homogenates, cell lysates and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: CD & Adhesion molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPR37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPR37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPR37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human GPR37 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3116MS