Human HMGA1(High Mobility Group AT Hook Protein 1) ELISA Kit

Product Overview

• Article Name: Human HMGA1(High Mobility Group AT Hook Protein 1) ELISA Kit
• Biozol Catalog Number: EBT-ELK3463
• Supplier Catalog Number: ELK3463
• Alternative Catalog Number: EBT-ELK3463-96, EBT-ELK3463-48, EBT-ELK3463-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Human
• Alternative Names: HMG-R, HMGIY, High mobility group protein A1, High mobility group protein R, High mobility group protein HMG-I/HMG-Y

Product Properties

• Concentration: 20 ng/mL
• Range: 0.32-20 ng/mL
• Sensitivity: 0.108 ng/mL
• UniProt: P17096
• Samples: tissue homogenates, cell lysates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Metabolic pathway,Tumor immunity,Developmental science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HMGA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMGA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HMGA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human HMGA1 in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK3463