Mouse HSP10(Heat Shock 10kDa Protein 1) ELISA Kit

Product Overview

• Article Name: Mouse HSP10(Heat Shock 10kDa Protein 1) ELISA Kit
• Biozol Catalog Number: EBT-ELK3599
• Supplier Catalog Number: ELK3599
• Alternative Catalog Number: EBT-ELK3599-96, EBT-ELK3599-48, EBT-ELK3599-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Mouse
• Alternative Names: HSPE1, CPN10, EPF, GROES, Chaperonin 10, Early Pregnancy Factor, 10 kDa heat shock protein, mitochondrial

Product Properties

• Concentration: 100 ng/mL
• Range: 1.57-100 ng/mL
• Sensitivity: 0.62 ng/mL
• Samples: Tissue homogenates, cell lysates and other biological fluids.

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Developmental science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse HSP10 in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK3599