Human FYN(FYN Oncogene Related To SRC/FGR/YES) ELISA Kit

Catalog Number: EBT-ELK3670
Article Name: Human FYN(FYN Oncogene Related To SRC/FGR/YES) ELISA Kit
Biozol Catalog Number: EBT-ELK3670
Supplier Catalog Number: ELK3670
Alternative Catalog Number: EBT-ELK3670-96, EBT-ELK3670-48, EBT-ELK3670-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: SLK, SYN, c-Fyn, Src-like kinase
Concentration: 10 ng/mL
Range: 0.16-10 ng/mL
Sensitivity: 0.06 ng/mL
Samples: Tissue homogenates, cell lysates and other biological fluids.
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FYN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FYN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FYN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human FYN in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3670