Human SOCS1(Suppressors Of Cytokine Signaling 1) ELISA Kit

Catalog Number: EBT-ELK3917
Article Name: Human SOCS1(Suppressors Of Cytokine Signaling 1) ELISA Kit
Biozol Catalog Number: EBT-ELK3917
Supplier Catalog Number: ELK3917
Alternative Catalog Number: EBT-ELK3917-96, EBT-ELK3917-48, EBT-ELK3917-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP3, JAK-binding protein, STAT-induced STAT inhibitor 1, Tec-interacting protein 3
Concentration: 20 ng/mL
Range: 0.32-20 ng/mL
Sensitivity: 0.116 ng/mL
UniProt: O15524
Samples: tissue homogenates, cell lysates and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SOCS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SOCS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SOCS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human SOCS1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK3917