Human WASF2(Wiskott Aldrich Syndrome Protein Family, Member 2) ELISA Kit

Catalog Number: EBT-ELK4267
Article Name: Human WASF2(Wiskott Aldrich Syndrome Protein Family, Member 2) ELISA Kit
Biozol Catalog Number: EBT-ELK4267
Supplier Catalog Number: ELK4267
Alternative Catalog Number: EBT-ELK4267-96, EBT-ELK4267-48, EBT-ELK4267-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: SCAR2, WAVE2, Verprolin homology domain-containing protein 2
Concentration: 20 ng/mL
Range: 0.32-20 ng/mL
Sensitivity: 0.105 ng/mL
UniProt: Q9Y6W5
Samples: Tissue homogenates and other biological fluids.
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Developmental science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WASF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WASF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WASF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human WASF2 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4267