Mouse IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit

Product Overview

• Article Name: Mouse IkBa(Inhibitory Subunit Of NF Kappa B Alpha) ELISA Kit
• Biozol Catalog Number: EBT-ELK4340
• Supplier Catalog Number: ELK4340
• Alternative Catalog Number: EBT-ELK4340-96, EBT-ELK4340-48, EBT-ELK4340-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Mouse
• Alternative Names: NFKBIA, IKB-A, MAD-3, NFKBI, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor,Alpha, Major histocompatibility complex enhancer-binding protein MAD3

Product Properties

• Concentration: 10 ng/mL
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• UniProt: Q9Z1E3
• Samples: Tissue homogenates, cell lysates and other biological fluids.

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Tumor immunity,Hematology,Developmental science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IkBa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IkBa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IkBa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse IkBa in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK4340