Human TNFSF9(Tumor Necrosis Factor Ligand Superfamily, Member 9) ELISA Kit

Catalog Number: EBT-ELK4364
Article Name: Human TNFSF9(Tumor Necrosis Factor Ligand Superfamily, Member 9) ELISA Kit
Biozol Catalog Number: EBT-ELK4364
Supplier Catalog Number: ELK4364
Alternative Catalog Number: EBT-ELK4364-96, EBT-ELK4364-48, EBT-ELK4364-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: 4-1BB-L, Receptor 4-1BB Ligand, Homolog Of Mouse 4-1BB-L
Concentration: 500 pg/mL
Range: 7.82-500 pg/mL
Sensitivity: 2.6 pg/mL
UniProt: P41273
Samples: serum, plasma, tissue homogenates and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFSF9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFSF9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFSF9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human TNFSF9 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4364