Rat L1CAM(L1-Cell Adhesion Molecule) ELISA Kit

Catalog Number: EBT-ELK4462
Article Name: Rat L1CAM(L1-Cell Adhesion Molecule) ELISA Kit
Biozol Catalog Number: EBT-ELK4462
Supplier Catalog Number: ELK4462
Alternative Catalog Number: EBT-ELK4462-96, EBT-ELK4462-48, EBT-ELK4462-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Rat
Alternative Names: CD171, L1-CAM, CAML1, HSAS, HSAS1, MASA, MIC5, N-CAML1, S10, SPG1, Antigen Identified By Monoclonal Antibody R1
Concentration: 5000 pg/mL
Range: 78.13-5000 pg/mL
Sensitivity: 25 pg/mL
UniProt: Q05695
Samples: Serum, plasma and other biological fluids.
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: CD & Adhesion molecule,Tumor immunity,Infection immunity,Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat L1CAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat L1CAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat L1CAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat L1CAM in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4462