Human HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit

Product Overview

• Article Name: Human HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit
• Biozol Catalog Number: EBT-ELK4514
• Supplier Catalog Number: ELK4514
• Alternative Catalog Number: EBT-ELK4514-96, EBT-ELK4514-48, EBT-ELK4514-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Human
• Alternative Names: DnaK, 70 Kilodalton Heat Shock Proteins,Heat shock 70 kDa protein 1, 2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1, HSP70 2, HSP70 1A, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B, HSP70, Heat Shock Protein 70

Product Properties

• Concentration: 200 ng/mL
• Range: 3.13-200 ng/mL
• Sensitivity: 1.39 ng/mL
• UniProt: P38646
• Samples: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Tumor immunity,Infection immunity,Developmental science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSP-70/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSP-70/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSP-70/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human HSP-70/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK4514