Human ACACa(Acetyl Coenzyme A Carboxylase Alpha) ELISA Kit

Catalog Number: EBT-ELK4541
Article Name: Human ACACa(Acetyl Coenzyme A Carboxylase Alpha) ELISA Kit
Biozol Catalog Number: EBT-ELK4541
Supplier Catalog Number: ELK4541
Alternative Catalog Number: EBT-ELK4541-96, EBT-ELK4541-48, EBT-ELK4541-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: ACAC, ACC1, ACCA, ACAC, ACC2, ACCB, ACACB, ACACA, Acetyl CoA Carboxylase, Biotin carboxylase
Concentration: 200 ng/mL
Range: 3.13-200 ng/mL
Sensitivity: 1.07 ng/mL
UniProt: Q13085
Samples: Serum, plasma and other biological fluids.
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACACa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACACa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACACa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human ACACa in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4541