Rabbit PPAR-gamma(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit

Product Overview

• Article Name: Rabbit PPAR-gamma(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
• Biozol Catalog Number: EBT-ELK4611
• Supplier Catalog Number: ELK4611
• Alternative Catalog Number: EBT-ELK4611-96, EBT-ELK4611-48, EBT-ELK4611-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Rabbit
• Alternative Names: PPAR-G, PPARG1, PPARG2, NR1C3, Glitazone Receptor, Nuclear Receptor Subfamily 1 Group C Member 3, PPARg, Peroxisome Proliferator Activated Receptor Gamma

Product Properties

• Concentration: 50 ng/mL
• Range: 0.79-50 ng/mL
• Sensitivity: 0.29 ng/mL
• UniProt: O19052
• Samples: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Endocrinology,Cardiovascular biology,Developmental science,Bone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit PPAR-gamma. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PPAR-gamma. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit PPAR-gamma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rabbit PPAR-gamma in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK4611