Alpha CTx, a-CTXI, aCTX-I, A-Cr, Type I Collagen C-Telopeptide-Related Fraction
Concentration:
5000 pg/mL
Range:
78.13-5000 pg/mL
Sensitivity:
25.7 pg/mL
Samples:
serum, plasma, other biological fluids
Application Notes:
Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Bone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human aCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human aCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human aCTx in the samples is then determined by comparing the OD of the samples to the standard curve
ELK4853
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