Human POLd(Polymerase DNA Directed Delta 1) ELISA Kit
Biozol Catalog Number:
EBT-ELK5175
Supplier Catalog Number:
ELK5175
Alternative Catalog Number:
EBT-ELK5175-96, EBT-ELK5175-48, EBT-ELK5175-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
CDC2, POLD1, DNA polymerase subunit delta p125
Concentration:
10 ng/mL
Range:
0.16-10 ng/mL
Sensitivity:
0.055 ng/mL
Samples:
Tissue homogenates, cell lysates and other biological fluids.
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human POLd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human POLd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human POLd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human POLd in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5175
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