Rat sIL-6R(SolubleInterleukin 6 Receptor) Microsample ELISA Kit

Catalog Number: EBT-ELK5684MS
Article Name: Rat sIL-6R(SolubleInterleukin 6 Receptor) Microsample ELISA Kit
Biozol Catalog Number: EBT-ELK5684MS
Supplier Catalog Number: ELK5684MS
Alternative Catalog Number: EBT-ELK5684MS-96, EBT-ELK5684MS-48, EBT-ELK5684MS-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Rat
Alternative Names: IL-6R,CD126, gp80, IL6R, IL-6R-1, IL-6R-Alpha, IL6RA, IL-6 receptor subunit alpha, Membrane glycoprotein 80, IL6R, Interleukin 6 Receptor
Concentration: 10 ng/mL
Range: 0.16-10 ng/mL
Sensitivity: 0.06 ng/mL
Samples: serum, plasma, tissue homogenates and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,CD & Adhesion molecule,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve
ELK5684MS