Chicken NF-kappaB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

Product Overview

• Article Name: Chicken NF-kappaB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
• Biozol Catalog Number: EBT-ELK5686
• Supplier Catalog Number: ELK5686
• Alternative Catalog Number: EBT-ELK5686-96, EBT-ELK5686-48, EBT-ELK5686-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Gallus
• Alternative Names: NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit, NFkB, Nuclear Factor Kappa B

Product Properties

• Concentration: 10 ng/mL
• Range: 0.16-10 ng/mL
• Sensitivity: 0.056 ng/mL
• UniProt: Q04861
• Samples: Tissue homogenates, cell lysates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken NF-kappaBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken NF-kappaBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken NF-kappaBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Chicken NF-kappaBp105 in the samples is then determined by comparing the OD of the samples to the standard curve

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