Pig TNFb(Tumor Necrosis Factor Beta) ELISA Kit

Product Overview

• Article Name: Pig TNFb(Tumor Necrosis Factor Beta) ELISA Kit
• Biozol Catalog Number: EBT-ELK5858
• Supplier Catalog Number: ELK5858
• Alternative Catalog Number: EBT-ELK5858-96, EBT-ELK5858-48, EBT-ELK5858-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Porcine
• Alternative Names: LT, LTA, TNF-B, TNFSF1, Tumor Necrosis Factor Ligand Superfamily Member 1, Lymphotoxin Alpha

Product Properties

• Concentration: 1000 pg/mL
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.6 pg/mL
• UniProt: P26445
• Samples: Serum, plasma and other biological fluids.

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig TNFb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig TNFb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig TNFb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Pig TNFb in the samples is then determined by comparing the OD of the samples to the standard curve

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