Dog NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit

Product Overview

• Article Name: Dog NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit
• Biozol Catalog Number: EBT-ELK6023
• Supplier Catalog Number: ELK6023
• Alternative Catalog Number: EBT-ELK6023-96, EBT-ELK6023-48, EBT-ELK6023-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Canine
• Alternative Names: NOS2A, INOS, HEP-NOS, I-NOS, Hepatocytes Oxide Synthase, Peptidyl-cysteine S-nitrosylase NOS2, NOS2, Nitric Oxide Synthase 2, Inducible

Product Properties

• Concentration: 20 ng/mL
• Range: 0.32-20 ng/mL
• Sensitivity: 0.131 ng/mL
• UniProt: O62699
• Samples: Serum, plasma, tissue homogenates and other biological fluids.

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,Metabolic pathway,Tumor immunity,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Dog NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK6023