Rat NPR1(Natriuretic Peptide Receptor 1) ELISA Kit

Catalog Number: EBT-ELK6852
Article Name: Rat NPR1(Natriuretic Peptide Receptor 1) ELISA Kit
Biozol Catalog Number: EBT-ELK6852
Supplier Catalog Number: ELK6852
Alternative Catalog Number: EBT-ELK6852-96, EBT-ELK6852-48, EBT-ELK6852-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Rat
Alternative Names: ANPRA, ANPa, GUC2A, GUCY2A, NPR-A, Guanylate Cyclase A, Atrionatriuretic Peptide Receptor A, Atrial natriuretic peptide receptor type A
Concentration: 20 ng/mL
Range: 0.32-20 ng/mL
Sensitivity: 0.105 ng/mL
UniProt: P18910
Samples: Tissue homogenates and other biological fluids.
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NPR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NPR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NPR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat NPR1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK6852