Simian ECF(Eosinophil Chemotactic Factor) ELISA Kit

Catalog Number: EBT-ELK7912
Article Name: Simian ECF(Eosinophil Chemotactic Factor) ELISA Kit
Biozol Catalog Number: EBT-ELK7912
Supplier Catalog Number: ELK7912
Alternative Catalog Number: EBT-ELK7912-96,EBT-ELK7912-48,EBT-ELK7912-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Primate
Alternative Names: CCL11, SCYA11, Eotaxin 1, Eosinophil Chemotactic Protein 1, Chemokine C-C-Motif Ligand 11, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11
Concentration: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sensitivity: 6.1 pg/mL
Samples: Serum, plasma and other biological fluids.
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Infection immunity,Autoimmunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian ECF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian ECF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian ECF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Simian ECF in the samples is then determined by comparing the OD of the samples to the standard curve