Human Anti-MBL(Anti-Mannose Binding Lectin Antibody) ELISA Kit

Catalog Number: EBT-ELK8057
Article Name: Human Anti-MBL(Anti-Mannose Binding Lectin Antibody) ELISA Kit
Biozol Catalog Number: EBT-ELK8057
Supplier Catalog Number: ELK8057
Alternative Catalog Number: EBT-ELK8057-96,EBT-ELK8057-48,EBT-ELK8057-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
Concentration: 400 ng/mL
Range: 6.25-400 ng/mL
Sensitivity: 2.54 ng/mL
Samples: Serum, plasma and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Infection immunity,Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MBL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human Anti-MBL in the samples is then determined by comparing the OD of the samples to the standard curve