Rat TPO-Ab(anti-Thyroid-Peroxidase antibody) ELISA Kit
Biozol Catalog Number:
EBT-ELK8352
Supplier Catalog Number:
ELK8352
Alternative Catalog Number:
EBT-ELK8352-96,EBT-ELK8352-48,EBT-ELK8352-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Rat
Alternative Names:
TPO Ab, TPO-Ab
Concentration:
80 IU/mL
Range:
1.25-80 IU/mL
Sensitivity:
1.25 IU/mL
Samples:
serum, plasma, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Signal transduction,Metabolic pathway,Infection immunity,Hormone metabolism,Dermatology,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve
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