Rabbit ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit
Biozol Catalog Number:
EBT-ELK8395
Supplier Catalog Number:
ELK8395
Alternative Catalog Number:
EBT-ELK8395-96,EBT-ELK8395-48,EBT-ELK8395-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Rabbit
Alternative Names:
Endocan
Concentration:
1000 pg/mL
Range:
15.63-1000 pg/mL
Sensitivity:
6 pg/mL
Samples:
serum, plasma, tissue homogenates, cell lysates and other biological fluids
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Tumor immunity,Infection immunity,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit ESM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit ESM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit ESM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rabbit ESM1 in the samples is then determined by comparing the OD of the samples to the standard curve
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