Dog sRANKL(Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit
Biozol Catalog Number:
EBT-ELK9165
Supplier Catalog Number:
ELK9165
Alternative Catalog Number:
EBT-ELK9165-96, EBT-ELK9165-48, EBT-ELK9165-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Canine
Alternative Names:
CD254, TNFSF11, ODF, OPGL, TRANCE, HRANKL2, SOdf, Tumor Necrosis Factor(ligand)superfamily Member 11, TNF-related activation-induced cytokine, RANkL, Receptor Activator Of Nuclear Factor Kappa B Ligand
Concentration:
1000 pg/mL
Range:
15.63-1000 pg/mL
Sensitivity:
5.5 pg/mL
Samples:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Metabolic pathway,Apoptosis,Tumor immunity,Bone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog sRANKL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog sRANKL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog sRANKL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Dog sRANKL in the samples is then determined by comparing the OD of the samples to the standard curve
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