Human MR-ProADM(Mid-regional pro-adrenomedullin) ELISA Kit

Catalog Number: EBT-ELK9597
Article Name: Human MR-ProADM(Mid-regional pro-adrenomedullin) ELISA Kit
Biozol Catalog Number: EBT-ELK9597
Supplier Catalog Number: ELK9597
Alternative Catalog Number: EBT-ELK9597-96, EBT-ELK9597-48, EBT-ELK9597-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: MR-ProADM
Concentration: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sensitivity: 9.17 pg/mL
Samples: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Endocrinology,Hormone metabolism,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MR-ProADM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MR-ProADM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MR-ProADM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human MR-ProADM in the samples is then determined by comparing the OD of the samples to the standard curve
ELK9597