Mouse pMAPT/pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit

Catalog Number: EBT-ELK9618
Article Name: Mouse pMAPT/pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit
Biozol Catalog Number: EBT-ELK9618
Supplier Catalog Number: ELK9618
Alternative Catalog Number: EBT-ELK9618-96, EBT-ELK9618-48, EBT-ELK9618-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Mouse
Alternative Names: pMAPT/pTAU
Concentration: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sensitivity: 9.29 pg/mL
Samples: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cell Biology,Cancer,Metabolism. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse pMAPT/pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse pMAPT/pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse pMAPT/pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse pMAPT/pTAU in the samples is then determined by comparing the OD of the samples to the standard curve
ELK9618