Human VISTA(V-type immunoglobulin domain-containing suppressor of T-cell activation) ELISA Kit

Product Overview

• Article Name: Human VISTA(V-type immunoglobulin domain-containing suppressor of T-cell activation) ELISA Kit
• Biozol Catalog Number: EBT-ELK9872
• Supplier Catalog Number: ELK9872
• Alternative Catalog Number: EBT-ELK9872-96, EBT-ELK9872-48, EBT-ELK9872-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Human
• Alternative Names: V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, Stress-induced secreted protein-1, Sisp-1, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor, C10orf54, SISP1, VISTA, PP2135, UNQ730, PRO1412, VSIR, VISTA

Product Properties

• Concentration: 1000 pg/mL
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.7 pg/mL
• UniProt: Q9H7M9
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cardiovascular,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VISTA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VISTA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VISTA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human VISTA in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK9872