Mouse Smad2/3 (Mothers Against Decapentaplegic Homolog 2/3) ELISA Kit
Biozol Catalog Number:
EBT-ELK9883
Supplier Catalog Number:
ELK9883
Alternative Catalog Number:
EBT-ELK9883-96, EBT-ELK9883-48, EBT-ELK9883-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Mouse
Alternative Names:
Smad 2/3
Concentration:
10 ng/mL
Range:
0.16-10 ng/mL
Sensitivity:
0.059 ng/mL
Samples:
serum, plasma, tissue homogenates
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Apoptosis,Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Smad2/3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Smad2/3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Smad2/3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse Smad2/3 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK9883
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