Recombinant Human LIF

Catalog Number: EBT-EPT153
Article Name: Recombinant Human LIF
Biozol Catalog Number: EBT-EPT153
Supplier Catalog Number: EPT153
Alternative Catalog Number: EBT-EPT153-10
Manufacturer: ELK Biotechnology
Category: Proteine/Peptide
Alternative Names: Leukemia Inhibitory Factor, LIF, Differentiation-Stimulating Factor, D Factor, Melanoma-Derived LPL Inhibitor, MLPLI, Emfilermin, LIF, HILDA
Recombinant Human Leukemia Inhibitory Factor is produced by our E.coli expression system and the target gene encoding Ser23-Phe202 is expressed.
Molecular Weight: Molecular weight: 19.7 KDa. Apparent molecular weight: 18 KDa, reducing conditions
UniProt: P15018
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Application Notes: Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.. Endotoxin: Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. Background: Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF