Cleaved-Caspase-4 p20 (Q81) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Cleaved-Caspase-4 p20 (Q81) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1019
• Supplier Catalog Number: ES1019
• Alternative Catalog Number: EBT-ES1019-50, EBT-ES1019-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 4. AA range:62-111
• Alternative Names: CASP4, ICH2, Caspase-4, CASP-4, ICE(rel)-II, Protease ICH-2, Protease TX

Product Description

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 43+22kD
• NCBI: 837
• UniProt: P49662

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody diluted at 1:2000

Western Blot analysis of 3T3 cells using Cleaved-Caspase-4 p20 (Q81) Polyclonal Antibody diluted at 1:2000

Western blot analysis of lysates from Jurkat cells, using Caspase 4 (p20,Cleaved-Gln81) Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).