The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys5. AA range:1-50
Alternative Names:
H2BFS, Histone H2B type F-S, Histone H2B.s, H2B/s,H2BK5AC
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Western Blot analysis of various cells using Acetyl-Histone H2B (K5) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog:RS0002) was diluted at 1:20000
Western Blot analysis of 3T3 cells using Acetyl-Histone H2B (K5) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog:RS0002) was diluted at 1:20000
Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys5) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys5) Antibody. The picture on the right is blocked with the synthesized peptide.
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