Histone H3 (Acetyl Lys23) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Histone H3 (Acetyl Lys23) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1089
• Supplier Catalog Number: ES1089
• Alternative Catalog Number: EBT-ES1089-50, EBT-ES1089-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys23. AA range:-9-40
• Alternative Names: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3,H3k23AC

Product Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 17kD
• NCBI: 8350
• UniProt: P68431

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Acetyl-Histone H3 (K23) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog:RS0002) was diluted at 1:20000

Western Blot analysis of L929 cells using Acetyl-Histone H3 (K23) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog:RS0002) was diluted at 1:20000

Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys23) Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys23) Antibody. The lane on the right is blocked with the synthesized peptide.