Histone H4 (Acetyl Lys8) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Histone H4 (Acetyl Lys8) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1092
• Supplier Catalog Number: ES1092
• Alternative Catalog Number: EBT-ES1092-50, EBT-ES1092-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys8. AA range:1-50
• Alternative Names: HIST1H4A, H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK,H4K8AC

Product Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 11kD
• NCBI: 121504
• UniProt: P62805

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Images

Immunofluorescence analysis of Hela cell. 1,Histone H4 (Acetyl Lys8) Polyclonal Antibody(red) was diluted at 1:200(4 overnight). HER2 Monoclonal Antibody(11H9)(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

Western Blot analysis of various cells using Acetyl-Histone H4 (K8) Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog:RS0002) was diluted at 1:20000

Western Blot analysis of KB cells using Acetyl-Histone H4 (K8) Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog:RS0002) was diluted at 1:20000

Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys8) Antibody. The picture on the right is blocked with the synthesized peptide.