NFkappaB-p65 (Acetyl Lys310) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: NFkappaB-p65 (Acetyl Lys310) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1094
• Supplier Catalog Number: ES1094
• Alternative Catalog Number: EBT-ES1094-50, EBT-ES1094-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the acetylated site of Lys310. AA range:275-324
• Alternative Names: RELA, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Product Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 65kD
• NCBI: 5970
• UniProt: Q04206

Application Notes

• Application Dilute: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300

Images

Immunofluorescence analysis of Hela cell. 1,NFkappaB-p65 (Acetyl Lys310) Polyclonal Antibody(red) was diluted at 1:200(4 overnight). beta-Tubulin Monoclonal Antibody(5G3)(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,NFkappaB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,NFkappaB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,NFkappaB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.