Ref-1 (Acetyl Lys7) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Ref-1 (Acetyl Lys7) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1098
• Supplier Catalog Number: ES1098
• Alternative Catalog Number: EBT-ES1098-50, EBT-ES1098-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized Acetyl-peptide derived from human APE1 around the Acetylation site of Lys7. AA range:1-50
• Alternative Names: APEX1, APE, APE1, APEX, APX, HAP1, REF1, DNA-(apurinic or apyrimidinic site) lyase, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1

Product Description

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5 to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene, all encode the same protein. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 35kD
• NCBI: 328
• UniProt: P27695

Application Notes

• Application Dilute: WB 1:500-2000,IHC-p 1:50-300

Images

Western Blot analysis of Jurkat, K562 cells using Acetyl-Ref-1 (K7) Polyclonal Antibody. Secondary antibody(catalog:RS0002) was diluted at 1:20000

Western Blot analysis of JK cells using Acetyl-Ref-1 (K7) Polyclonal Antibody. Secondary antibody(catalog:RS0002) was diluted at 1:20000

Western blot analysis of lysate from Jurkat cells, using APE1 (Acetyl-Lys7) Antibody.

Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).