The antiserum was produced against synthesized peptide derived from human Akt2 around the phosphorylation site of Ser474. AA range:432-481
Alternative Names:
AKT2, RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Western Blot analysis of various cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1:500
Western Blot analysis of 293 cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1:500
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Akt2 (Phospho-Ser474) Antibody
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Akt2 (Phospho-Ser474) Antibody. The picture on the right is blocked with the phospho peptide.
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