ATF-2 (phospho Thr71) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: ATF-2 (phospho Thr71) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1267
• Supplier Catalog Number: ES1267
• Alternative Catalog Number: EBT-ES1267-50, EBT-ES1267-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human ATF2 around the phosphorylation site of Thr71 or 53. AA range:38-87
• Alternative Names: ATF2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding pro

Product Description

activating transcription factor 2(ATF2) Homo sapiens This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro, thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cells DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 52kD
• NCBI: 1386
• UniProt: P15336

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-ATF-2 (T71) Polyclonal Antibody diluted at 1:1000

Western Blot analysis of HuvEc cells using Phospho-ATF-2 (T71) Polyclonal Antibody diluted at 1:1000

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ATF2 (Phospho-Thr71 or 53) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with UV, using ATF2 (Phospho-Thr71 or 53) Antibody. The lane on the right is blocked with the phospho peptide.