Cdc25B (phospho Ser323) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: Cdc25B (phospho Ser323) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1283
• Supplier Catalog Number: ES1283
• Alternative Catalog Number: EBT-ES1283-50, EBT-ES1283-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDC25B around the phosphorylation site of Ser323. AA range:289-338
• Alternative Names: CDC25B, CDC25HU2, M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B

Product Description

cell division cycle 25B(CDC25B) Homo sapiens CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 70kD
• NCBI: 994
• UniProt: P30305

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-Cdc25B (S323) Polyclonal Antibody diluted at 1:1000

Western Blot analysis of VEC cells using Phospho-Cdc25B (S323) Polyclonal Antibody diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDC25B (Phospho-Ser323) Antibody