c-Src (phospho Tyr529) rabbit pAb, Rabbit, Polyclonal

Product Overview

• Article Name: c-Src (phospho Tyr529) rabbit pAb, Rabbit, Polyclonal
• Biozol Catalog Number: EBT-ES1293
• Supplier Catalog Number: ES1293
• Alternative Catalog Number: EBT-ES1293-50, EBT-ES1293-100
• Manufacturer: ELK Biotechnology
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human Src around the phosphorylation site of Tyr529. AA range:487-536
• Alternative Names: SRC, SRC1, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src

Product Description

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Molecular Weight: 59kD
• NCBI: 6714
• UniProt: P12931

Application Notes

• Application Dilute: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Images

Western Blot analysis of various cells using Phospho-c-Src (Y529) Polyclonal Antibody diluted at 1:2000

Western Blot analysis of COS7 cells using Phospho-c-Src (Y529) Polyclonal Antibody diluted at 1:2000

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Src (Phospho-Tyr529) Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Src (Phospho-Tyr529) Antibody. The picture on the right is blocked with the phospho peptide.